【摘要】 目的:建立高效液相色谱-质谱/质谱联用测定人血浆中赖诺普利浓度的方法,用于赖诺普利口腔崩解片的人体相对生物利用度和生物等效性研究。方法:使用Hypurity C18柱(2.1mm×150mm,5μm),以0.1%甲酸-乙腈(30∶70)为流动相,流速为0.2mL·min-1,柱温为40℃。以二级质谱选择离子反应监测方式(SRM)扫描检测。结果:赖诺普利的线性浓度范围为1.64～210ng·mL-1(r=0.9915),最小检出浓度为0.82ng·mL-1。提取回收率大于50%,平均相对回收率在80%～120%范围内。日内与日间变异均小于15%。结论:本法简便、灵敏,可用于赖诺普利的药物代谢动力学和生物等效性研究。更多还原

【Abstract】 Objective:To establish a method of HPLC-MS/MS to detect lisinopril tablets in human plasma.Methods:Chromatographic column was Hypurity C18 (2.1 mm×150 mm,5 μm);The mobile phase was 0.1% formic acid-acetonitrile (30∶70);The flow rate was 0.2 mL·min-1 and temperature of column was 40 ℃.A MS/MS selective reaction monitoring mode was taken to quantitate lisinopril in human plasma.Results:Standard curve was linear in the range of 1.64-210 ng·mL-1 (r=0.9915).The lower limit of detections of lisinopril was 0.82 ng·mL-1.The absolute recovery was more than 50%,and the relative recoveries of lisinopril were in the range of 80%-120%.Relative standard deviations of intraday and interday assays of lisinopril were both less than 15%.Conclusion:The method is convenient and sensitive for detecting the concentration of lisinopril in human plasma,it can be used to study the pharmacokinetics and bioequivalence of lisinopril.更多还原