【摘要】 目的:建立一种快速准确检测大鼠肝微粒体中白花前胡甲素(Pd-Ia)含量的方法。方法:以华蟾毒精为内标物,采用Hypersil-C18(250mm×4.6mm,5μm)色谱柱,以甲醇-水(75∶25)为流动相,流速1mL·min-1,检测波长321nm,柱温25℃,进样量20μL。结果:Pd-Ia浓度在1～100μg·mL-1范围内与峰面积呈现良好的线性关系(r=0.9989);日内精密度RSD≤2.9%;日间精密度RSD≤8.5%;平均加样回收率(n=9)为98.4%。结论:本文建立的方法操作简单,可用于Pd-Ia含量的测定。更多还原

【Abstract】 Objective:To establish a method of determination of praeruptrum A(Pd-Ia)in rat hepatic microsomes fraction by HPLC.Method:Hypersil-C18(250 mm × 4.6 mm,5 μm) column was adopted at a temperature of 25 ℃.An HPLC method was established using cinobufagin as internal standard.The mobile phase consisted of methanol-water (75∶25) at a flow rate of 1.0 mL·min-1.The UV detection wavelength was set at 321 nm,the injection volume was 20 μL.Results:The calibration curve was shown to be a linear over the range of 1-100 μg·mL-1 (r=0.9989).The mean recovery(n=9) was 98.4%,The RSDs of intra-day and inter-day were less than 2.9% and 8.5%,respectively.Conclusion:The method is specific,simple and has been successfully applied to the determination of Pd-Ia.更多还原