【摘要】 目的:对急性子中2种主要凤仙萜四醇苷类成分凤仙萜四醇苷K、凤仙萜四醇苷A进行含量测定。方法:采用反相高效液相色谱-蒸发光散射检测法,使用Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-水(梯度洗脱,0～22min时为50∶50,22～50min时为54∶46),流速0.8mL·min-1,柱温25℃。ELSD检测器条件:载气(空气)流速2.5L·min-1,漂移管温度89℃。结果:凤仙萜四醇苷K、A进样量在5～20μg范围内线性关系良好,相关系数(n=6)分别为0.9995和0.9992;平均回收率(n=6)均为98.0%,RSD均为1.3%。结论:本方法能够准确测定急性子中凤仙萜四醇苷K、A的含量,方法简便,结果可靠。更多还原

【Abstract】 Objective:To determine two hosenkosides(hosenkoside K and hosenkoside A)in the seeds of Impatiens balsamina.Methods:Using RP-HPLC with ELSD method.Separation was carried out on Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm).A linear solvent gradient was used,with a mobile phase of methanol-water(0-22 min,50∶50;22-50 min,54∶46).The flow rate was 0.8 mL·min-1 at 25 ℃.The gas(air)flow rate was 2.5 L·min-1 and the temperature of drift tube for measurement was 89 ℃.Results:The linearity was in the range of 5-20 μg for hosenkoside K and A,and the correlation coefficients(n=6)were 0.9995 and 0.9992 respectively;The average recoveries(n=6)for hosenkoside K and A were 98.0%(RSD=1.3%)and 98.0%(RSD=1.3%)respectively.Conclusion:This method is accurate,reliable and reproducible for determination of hosenkoside K and A in the seeds of Impatiens balsamina.更多还原