【摘要】 目的:建立固相萃取-毛细管区带电泳定量检测肉类中5种磺胺类药物残留。方法:样品经乙腈提取后,固相萃取浓缩;75μm×57cm(有效长度50cm)毛细管,0.02mol·L-1磷酸盐(pH8.0)缓冲溶液,分离电压20kV,温度25℃,2.7kPa进样15s,检测波长254nm。结果:磺胺地索辛,磺胺噻唑,磺胺嘧啶,磺胺二甲嘧啶,磺胺甲恶唑8min内基线分离,0.4～40.0μg·mL-1范围内线性关系良好(r>0.9983),检测限0.05～0.10μg·mL-1,样品加标回收率70.6%～105.1%。结论:该方法简单、快速、灵敏度高、重现性好。更多还原

【Abstract】 Objective:To establish a solid phase extraction-capillary zone electrophoresis method for quantitative determination of sulfonamides in meat.Method:Samples were extracted by acetonitrile and solid phase extraction columns were used for cleaning up.Using untreated fused silica capillary (75 μm×57 cm,effective length was 50 cm) as separation column,0.02 mol·L-1 phosphate (pH 8.0) as buffer,separation voltage was 20 kV,temperature was 25 ℃,samples were injected at 2.7 kPa for 15 s,and detected at 254 nm.Results:Sulfadimethoxine (SDM),sulfathizole (STZ),sulfadiazine (SDZ),sulfamethazine (SMZ) and sulfamethoxazole (SMX) were baseline separated within 8.0 min.The calibration curves were linear in the range of 0.4-40.0 μg·mL-1(r>0.9983).The detection limits were 0.05-0.10 μg·mL-1.The recoveries of the sulfonamides were 70.6%-105.1%.Conclusion:This method is simple,rapid,sensitive and reproducible.更多还原