【摘要】 目的:建立人血浆中多沙唑嗪浓度的高效液相色谱-荧光检测方法。方法:以特拉唑嗪为内标。血浆样品经碱化后用乙醚萃取。采用Supelcosil ABZ+PLUS C18色谱柱(250 mm×4.6 mm,5μm),20 mmol·L-1磷酸盐缓冲液-乙腈(67∶33)为流动相,流速为1.20 mL·min-1,荧光检测波长为λex=335 nm,λem=390 nm,进样量为50μL。结果:在1.0～50.0μg·L-1浓度范围内,测定方法具良好的线性关系,线性方程为:A=0.1639C-0.0253(r=0.9998),准确度和精密度符合生物样品分析要求,最低定量浓度为1.0μg·L-1。结论:本测定方法具灵敏度高、准确和快速的优点,可用于甲磺酸多沙唑嗪人体药代动力学和生物等效性研究。更多还原

【Abstract】 Objective:To establish a simple,sensitive and rapid RP-HPLC with fluorescence detection method for determination of doxazosin in human plasma.Methods:Terazosin was used as the internal standard.The plasma samples were extracted with ether.The solvent was evaporated to dryness at 40 ℃ in water bath.The Supelcosil ABZ+PLUS C18column(250 mm×4.6 mm,5 μm) was used analytical column.The mobile phase consisted of 20 mmol·L-1 potassium dihydrogenphosphate solution-acetonitrile(67∶33) with a flow rate of 1.20 mL·min-1.The fluorescence detector were set at λex=335 nm,λem=390 nm.The injection volume was 50 μL.Results:A good linear relationship was obtained between the peak area ration of doxazosin/terazosin and the concentration of doxazosin over the rang of 1.0 to 50.0 μg·L-1.The linear equation was A=0.1639C-0.0253(r=0.9998).The limit of quantitative concentration was 1.0 μg·L-1.Conclusion:The method is sensitive,accurate and simple for the determination of doazosin in human plasma.It is suitable for the pharmacokinetic and bioequivalence studies of doxazosin in human.更多还原