【摘要】 目的:测定西洋参根及茎叶皂苷提取物中12种单体人参皂苷的含量。方法:采用WatersAlliance2690液相色谱仪,配Waters996型-PDA检测器,XTerraRP18色谱柱(3.9mm×150mm,5μm),乙腈(A)-水(B)为流动相,梯度洗脱[0min(20%A)→20min(20%A)→25min(25%A)→45min(26%A)→55min(27%A)→70min(32%A)→80min(47%A)→85min(53%A)→90min(53%A)],流速为1.0mL.min-1,检测波长203nm,柱温40℃。结果:12种人参皂苷Rg1、Rg2、Rg3、Re、Rd、Rh1、Rh2、Rf、Rc、Rb1、Rb2和Rb3浓度在其各自的线性范围内,与峰面积呈良好的线性关系,r值均>0.99(n=6);回收率在91.0%～100.4%范围内。结论:本方法精密度和重现性良好,适用于西洋参根及茎叶皂苷提取物中的单体人参皂苷的分析研究,可用于控制西洋参根及茎叶皂苷提取物的质量。更多还原

【Abstract】 Objective:To establish an RP-HPLC method for determination of the contents of twelve active constituents ginsenoside Rg1,Rg2,Rg3,Re,Rd,Rh1,Rh2,Rf,Rc,Rb1,Rb2 and Rb3,in extract of root,and stem and leaf from Panax quinquefolius.Method:The separating was performed on a XTerra RP18 column(3.9 mm×150 mm,5 μm).The mobile phase was acetonitrile(A)-water(B),with gradient elution [0 min(20% A)→20 min(20% A)→25 min(25% A)→45 min(26% A)→55 min(27% A)→70 min(32% A)→80 min(47% A)→85 min(53% A)→90 min(53% A)] at a flow-rate of 1.0 mL·min-1.Detection was at 203 nm,and the column temperature was set up at 40 ℃.Results:Rg1,Rg2,Rg3,Re,Rd,Rh1,Rh2,Rf,Rc,Rb1,Rb2and Rb3had good linear relation in their linear ranges(r≥0.99),the average recoveries were between 91.0%-100.4%.Conclusion:This method is accurate and reproducible as well as specific and can assess the quality of extract of root,and stem and leaf from Panax quinquefolius.更多还原