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短葶山麦冬药材定性与定量分析方法研究

Studies on the quality and quantity analysis methods of Liriope muscari(Decne.)Baily

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【作者】 张萍张南平汗克孜程显隆肖新月林瑞超

【Author】 ZHANG Ping1,ZHANG Nan-ping1,HAN Ke-zi2,CHENG Xian-long1,XIAO Xin-yue1,LIN Rui-chao1(1.National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China;2.Xinjiang Kashi Institute for Drug Control,Kashi 844000,China)

【机构】 中国药品生物制品检定所新疆喀什地区药品检验所

【摘要】 目的:建立短葶山麦冬药材主要药用成分的定性与定量分析方法。方法:采用薄层色谱法对短葶山麦冬药材中甾体皂苷类成分进行定性鉴别;采用紫外-可见分光光度法对短葶山麦冬药材中多糖成分进行含量测定,以苯酚-硫酸显色,在490nm的波长处测定吸收度。结果:应用所建立的方法对14批药材进行定性与定量分析,结果在TLC图谱中均可检出短葶山麦冬药材的特征斑点;14批样品多糖含量测定结果以无水葡萄糖计为6.34%~13.69%。结论:本方法操作简便,重复性好,结果准确可靠,可用于该药材的定性与定量分析。

【Abstract】 Objective:To establish the quality and quantity analysis methods of Liriope muscari(Decne.)Baily.Method:With TLC to identify steroid glycoside of Liriope muscari(Decne.)Baily;Ultraviolet spectrophotometry and colourimetry was used to determinate polysaccharide contents of Liriope muscari(Decne.)Baily,and the detected wavelength was 490 nm.Result:14 samples were determinated by the new method.The characteristic spots of Liriope muscari(Decne.)Baily could be easily examined by TLC.The polysaccharide contents of Liriope muscari(Decne.)Baily were 6.34%-13.69%.Conclusion:These methods are simple,accurate and can be used for the quality and quantity analysis of Liriope muscari(Decne.)Baily.

  • 【文献出处】 药物分析杂志 ,Chinese Journal of Pharmaceutical Analysis , 编辑部邮箱 ,2009年01期
  • 【分类号】R284.1
  • 【被引频次】10
  • 【下载频次】506
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