【摘要】 目的:建立测定中药和保健食品中低聚果糖(包括蔗果三糖、蔗果四糖、蔗果五糖3种成分)的高效液相含量测定方法。方法:色谱柱Agilent zorbax NH2(4.6 mm×250mm,5μm),柱温25℃;流动相水-乙腈(32:68),流速1.2mL.min-1;视差折光检测器,内部温度35℃。结果:蔗果三糖、蔗果四糖、蔗果五糖的线性范围分别为2.26~22.6μg,2.16~21.6μg,2.10~21.0μg;加样回收率(n=5)分别为96.32%,96.98%,95.67%。结论:本方法简便、灵敏、准确,可用于含低聚果糖中药和保健食品中以上3种成分的含量测定和产品质量控制。更多还原

【Abstract】 Objective:To develop an RP-HPLC-RID method for determination of fructooligosaccharides(including kestose,nystose,Fructofranosylnystose) in chinese medicine and functional foods.Methods:An Agilent zorbax NH2 column(4.6 mm×250mm,5μm)was applied;a mobile phase composing of acetonitrile and water(68:32) was used,and the flow rate was 1.2 mL·min-1;A RID detector was applied,and the inner temperature was set at 35 ℃.Results:The results were obtained that quantitative line range of three fructooligosaccharides was 2.26～22.6μg,2.16～21.6μg,and 2.10～21.0μg respectively,and the average recovery(n=5) was 96.32%,96.98%,and 95.67%,respectively.Conclusion:A specific,sensitive,simple and reproducible RP-HPLC-RID method was established for the determination of the three ingredients in chinese medicine and functional foods that claimed containing fructooligosaccharides.更多还原