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在线自校正激光诱导荧光检测微芯片的应用
Application of Microchip with Auto-deducting Background Diode Laser Induced Fluorescence Detector
【摘要】 实验室自行搭建完成一套微芯片体系,包括整体式聚二甲基硅氧烷(PDMS)微芯片及基于电荷耦合装置(CCD)的在线自校正激光诱导荧光检测装置。该体系具有微通道清洗及进样操作简单、分离快速等特点,已成功运用于氨基酸分离及标准蛋白质的筛分。
【Abstract】 A micro-separation system was established,including an integrated poly(dimethylsiloxane) microchip and on-line auto-deducting background diode laser induced fluorescence detector based on charge coupling devices(CCD).Virtues of the micro-separation system include simple operation such as channel cleaning and sample injecting and fast separation.The micro-separation system has been used for separation of amino acid and standard proteins.
【关键词】 微芯片;
自校正激光诱导荧光检测;
氨基酸;
蛋白质;
【Key words】 microchip; auto-deducting background diode laser induced fluorescence detector; amino acid; protein;
【Key words】 microchip; auto-deducting background diode laser induced fluorescence detector; amino acid; protein;
- 【文献出处】 延安大学学报(自然科学版) ,Journal of Yanan University(Natural Science Edition) , 编辑部邮箱 ,2009年01期
- 【分类号】Q503
- 【下载频次】79