【摘要】 此文采用722S型可见分光光度计,以连二亚硫酸钠为还原剂,对传统TTC-脱氢酶活性测定法中影响标准曲线制作的因素进行了研究,确定了最佳测定条件为:最大吸收峰在475nm处;连二亚硫酸钠的最佳投量为5.0mg/5mL;TTC溶液的保存及整个实验过程应避光。最佳反应时间为30min。更多还原

【Abstract】 In this paper the factors which influence the standard curve of determination are investigated by using 722S type visible pectrophotometer and sodium hydrosulfite as reductant in the measure method conventional TTC-Dehydrogenase activity.The optimum conditions are as follows:the maximum absorption peak is at the point of 475 nm;the best dosage of sodium dithionite is 5.0 mg/5mL ;preservation of TTC solution and the whole test process should be in darkness;the optimum reaction time is 30 min.更多还原