【摘要】 利用植物生物反应器生产外源药用蛋白近年来备受关注,本研究通过农杆菌介导法,将人工合成的鸡α干扰素基因(ChIFN-α)转化烟草(Nicotiana tabacum)无菌苗叶盘。对抗性植株进行的GUS活性鉴定,PCR和RT-PCR检测表明,ChIFN-α基因已整合到烟草基因组中并具有转录活性,ELISA检测和细胞病变(CPE)抑制试验表明转基因烟草表达的干扰素蛋白具有抗病毒活性。更多还原

【Abstract】 Expression of avian cytokine in tobacco or other plants had the economically potential use for producing pharmacological applications products and applying easily in practice.The cDNA encoding chicken alpha interferon(ChIFN-α)was introduced into Nicotiana tabacum Xanthin by Agrobacterium transformation.The transgenic plants were detected by histochemical stain assay and molecular analysis.Recombinant ChIFN-α was confirmed by enzyme-linked immunosorbent assay(ELISA)and cytopathic effect(CPE)inhibition assay.The results showed that ChIFN-α gene was successfully introduced into tobacco plants and stably expressed at various level from 0.0003% to 0.0033% of total soluble protein.The biological activity of ChIFN-α was 5.01×103 IU/g of tissue in vitro.Since interferon was involved in host antiviral response,our results provided the possibility for further study of plant oral adjuvant with ChIFN-α for improving immunity in birds.更多还原