【摘要】 我们观测了不同光照预处理对拟南芥、小麦和大豆叶片光合作用和低温(77K)叶绿素荧光参数F685、F735和F685/F735的影响。野生型拟南芥叶片光合作用对饱和光到有限光转变的响应曲线是V型,而缺乏叶绿体蛋白激酶的突变体STN7的这一曲线为L型。饱和白光可以引起拟南芥叶片F685/F735的明显降低,但是F735没有明显增高,而弱红光可以导致拟南芥叶片F685/F735的明显降低和F735的明显增高,表明弱红光可以引起状态1向状态2的转变,同时伴随从光系统Ⅱ脱离的LHCⅡ与光系统I的结合,而饱和白光只能引起LHCⅡ从光系统Ⅱ反应中心复合体脱离。并且,低温叶绿素荧光分析结果证明,饱和白光可以引起大豆叶片LHCⅡ脱离,但是不能引起小麦叶片LHCⅡ脱离,而弱红光可以引起小麦叶片的这种状态转换,却不能引起大豆叶片的这种状态转换。因此,饱和白光引起的野生型拟南芥和大豆叶片的LHCⅡ脱离不是一个典型的状态转换现象。更多还原

【Abstract】 The effects of different irradiance pre-treatments on leaf photosynthesis and low temperature(77K) chlorophyll fluorescence(LTCF) parameters F685,F735,and F685/F735 were observed in Arabidopsis,wheat,and soybean leaves.The curve of photosynthetic responsing to irradiance transition from saturating to limiting one in Arabidopsis wild-type leaves was the V pattern,while the curve in Arabidopsis mutant lacking chloroplast protein kinase STN7 was the L pattern.Saturating white irradiation(SWI) could induce the significantly decreased F685/F735 without a significantly increased F735,while weak red irradiation(WRI) could lead to the significantly declined F685/F735 with a significantly increased F735.The results showed that the attachment of dissociated LHC II to PS I in Arabidopsis wild-type leaves,indicating that WRI can cause the transition from state 1 to sate 2,while SWI can only induce LHC II dissociation from PSII reaction center complex.Moreover the results of LTCF analysis demonstrated that SWI could induce the LHC II dissociation in soybean but not wheat leaves,while WRI could cause the state transition in wheat but not soybean leaves.Hence the LHC II dissociation caused by SWI in Arabidopsis wild-type and soybean leaves was not a typical phenomenon of the state transition.更多还原