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瑞氏木霉β-内切葡聚糖酶的纯化及其部分酶学性质研究

Purification of β-endoglucanase in T. reesei QM9414 and its enzymological study

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【作者】 伍红农向张琪秦天莺黄惟巍谭德勇

【Author】 WU Hong1,NONG Xiang2,ZHANG Qi1,QIN Tian-ying1,HUANG Wei-wei3,TAN De-yong3(1.College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China;2.College of Chemistry and Life Science,Leshan Normal University,Leshan 614000,China;3.School of Life Science,Yunnan University,Kunming 650091,China)

【机构】 西南民族大学生命科学与技术学院乐山师范学院化学与生命科学学院云南大学生命科学学院

【摘要】 利用盐析、SephadexG-75和DEAE-SephadexA50层析,从瑞氏木霉Trichoderma reeseiQM9414发酵液中纯化了2个具有β-内切葡聚糖酶活性的组分Eg1和Eg2,分子质量分别为65.47 ku和57.04 ku.其最适温度分别为50℃和55℃,最适pH分别为4.8和5.0,米氏常数Km分别为3.76×10-2g/L和4.20×10-2g/L.根据其酶学性质,这是一类不同于已有的β-内切葡聚糖酶,为瑞氏木霉T.reesei QM9414所产β-内切葡聚糖酶的进一步研究奠定了基础.

【Abstract】 Two active components Eg1 and Eg2 of β-endoglucanase were purified by utilizing salting out,Sephadex G-75 chromatography and DEAE-Sephadex A50 chromatography from the fermentation broth of Trichoderma reesei QM9414.The relative molecular weights of Eg1 and Eg2 were 65.47ku and 57.04ku respectively;The optimal temperatures of Eg1 and Eg2 were 50℃ and 55℃ respectively;the optimal pH was 4.8 and 5.0 and their Michaelis constants were Km=3.76×10-2g/L and Km=4.20×10-2g/L.According to their enzymological characters,they are newly discovered.It seemed that the research built a basis for the further study of β-endoglucanase in T.reesei QM9414.

【基金】 西南民族大学自然科学基金项目资助(23444)
  • 【文献出处】 云南大学学报(自然科学版) ,Journal of Yunnan University(Natural Sciences Edition) , 编辑部邮箱 ,2009年03期
  • 【分类号】Q78
  • 【被引频次】2
  • 【下载频次】267
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