【摘要】 根据GenBank中鸡α干扰素(IFN-α)基因序列设计1对引物,应用PCR技术直接从鸡肝组织基因组中扩增罗曼鸡、海兰鸡及固始鸡α干扰素基因,并进行克隆和测序。结果表明,获得了3个品种鸡IFN-α全基因序列,长度均为582 bp,编码193个氨基酸残基。3个品种鸡IFN-α基因及推导的氨基酸序列比较结果显示,海兰鸡和罗曼鸡IFN-α基因序列完全一致,而固始鸡与海兰鸡和罗曼鸡IFN-α基因核苷酸同源性99.3%,有4个碱基发生非同义变异,氨基酸同源性为97.9%。克隆的3个品种鸡IFN-α基因序列与Gen-Bank上发表的7条鸡IFN-α基因序列进行比较分析发现,核苷酸同源性在98.1%~100%之间,氨基酸同源性在96.9%~100%之间。更多还原

【Abstract】 One pair of primers was designed according to chicken interferon-alpha(IFN-α)gene sequences retrieved from the GenBank.Chicken IFN-α genes were amplified from genome DNA extracted directionally from α livers of Guishi chicken,Hailan chicken and Luoman chicken.The purified PCR products were cloned into pGEM-T Easy vector and sequenced.The result showed that the full length sequence of chicken IFN-α gene was consisted of 582 bp,which includes one open-reading frame(582 bp),encoding 193 amino acid residues.When the nucleotide sequences of three line chicken IFN-α genes were compared,the nucleotide sequence of Hailan chicken IFN-α gene was just the same as Luoman chicken IFN-α gene,the nucleotide sequence of Guishi chicken IFN-α shared 99.3% homology with that of Hailan chicken and Luoman chicken IFN-α,and putative amino acid sequence shared 97.9%.When three line chicken IFN-α genes were compared with seven chicken IFN-α genes published previously in GenBank,there was identity of 98.1%~100%at nucleotide acid level,and that of 96.9%~100% separately at deduced amino acid level.更多还原