【摘要】 目的探讨整合素α5β1及黏着斑激酶(FAK)在慢性乙型肝炎肝纤维化形成中的作用。方法纳入慢性乙肝患者100例,其中S0期20例,S1期21例,S2期22例,S3期19例,S4期18例。均行肝组织穿刺,采用S-P免疫组化染色观察肝组织整合素α5β1、FAK的表达分布,并采用HPIAS-1000型多媒体真彩色病理图文分析系统进行半定量测定。其含量以表达阳性区域占全视野面积的百分比(面密度)平均数表示。结果肝组织整合素α5β1、FAK染色阳性物质呈棕黄色,其含量随着纤维化程度(S0～S4)的加重而逐渐增加(F值分别为58.87、59.14,P<0.05);整合素α5β1与FAK表达呈明显正相关(r=0.711,P<0.01)。结论整合素α5β1及FAK在慢性乙型肝炎肝纤维化形成中起着重要作用。更多还原

【Abstract】 Objective To explore the mechanism of integrin α5β1 and focal adhesion kinase(FAK)in hepatic fibrosis caused by chronic hepatitis B.Methods Totally 100 patients with chronic hepatitis B were enrolled,including 20 cases in S0,21 cases in S1,22 cases in S2,19 cases in S3,and 18 cases in S4.Through hepatic puncture,the expression and distribution of integrin α5β1 and FAK in liver tissue was detected by SP immunohistochemical staining,and semi-quantitatively determining them with HPIAS-1000 Multimedia True Color pathological analysis system.Their contents were presented by average percentages,which was ratios of the positive areas to the entire region(surface density).Results The positive stain of integrin α5β1 and FAK in liver tissue was presented with buffy-yellow color,and the content increased gradually with the degree of fibrosis raised(F=58.87,59.14 respectively,P<0.05).The expression of integrin α5β1 had positive correlation with expression of FAK(r=0.711,P<0.01).Conclusion It proved that the expression of integrin α5β1 and FAK played important roles in the promotion of hepatic fibrosis caused by chronic hepatitis B.更多还原