【摘要】 目的:探讨甘草酸二铵磷脂复合物注射剂(DG-PC)对C57BL/6J小鼠慢性免疫性肝脏损害的保护作用。方法:复制C57BL/6J小鼠慢性免疫性肝脏损害模型,测定血清ALT、AST、蛋白和透明质酸(HA),肝匀浆中超氧化物岐化酶(SOD)、谷胱甘肽(GSH)、I型胶原(C.I),并对肝脏组织切片进行电镜观察。结果:DG-PC可以明显改善肝损小鼠AST水平、提高白蛋白(ALB)含量、升高白球比(A/G)、降低HA含量(P<0.01),提高肝损小鼠的SOD和GSH水平(P<0.01),治疗效果优于甘草酸二铵(DG)。小鼠肝组织透射电镜观察显示DG-PC可以保护肝细胞和细胞器,减轻纤维化程度。结论:DG-PC对于C57BL/6J小鼠慢性免疫性肝损伤均具有一定的保护作用,可能与抗氧化等活性有关。更多还原

【Abstract】 AIM: To approach the protective effects of diammonium glycyrrhizinate phospholipid complex injection(DG-PC) on chronic and immunological liver injury.METHODS: The chronic and immunological hepatic liver injury was established in C57BL/6J mice,the levels of ALT,AST,TP,ALB and HA in serum were determined,the contents of SOD,GSH and collagen 1(C.I) in liver homogenate were detected,and the liver tissue sections were observed by electron microscope.RESULTS: Compared with DG,DG-PC could significantly improve the AST level,increase the content of ALB,increase the A/G,decrease the content of HA(P<0.01),improve the activities of SOD and GSH(P<0.01).The therapeutic efficacy of DG-PC was better than that of DG.As to microstructure,DG-PC could protect the hepatic cell and organelle,and relieve the fibrosis level.CONCLUSION: DG-PC has protective effects on chronic and immunological liver damage to C57BL/6J mice,which maybe relate to the antioxidant effects.更多还原