【摘要】 目的:观察丹酚酸胶囊的抗心肌缺血作用。方法:56只体重为(12±2)kg的健康Beagle犬,雌雄兼用,随机分为假手术组,模型对照组、高(100 mg/kg)、中(50 mg/kg)、低(25 mg/kg)剂量的丹酚酸胶囊组,丹参片组(275 mg/kg),地奥心血康胶囊组(100 mg/kg)。采用结扎冠状动脉前降支法致Beagle犬急性心肌缺血,经十二指肠给药后,用心外膜电图标测法计算心肌缺血程度,硝基四氮唑蓝(NBT)组织化学染色法测定心肌梗死面积,检测血清中磷酸肌酸激酶(CK)和乳酸脱氢酶(LDH),观察丹酚酸胶囊对心肌缺血损伤的保护作用。结果:丹酚酸胶囊可明显降低心肌缺血程度(∑-ST),减少心肌缺血范围(N-ST),缩小心肌梗死面积(MIS),并能显著降低血清CK和LDH活力,明显减轻心肌细胞损伤程度。结论:丹酚酸胶囊对急性心肌缺血犬具有明显的保护作用。更多还原

【Abstract】 AIM: To observe the effect of salvianolic acid capsule on acute myocardial ischemia in Beagle dogs.METHODS: 56 Beagle dogs weighting(12±2) kg were randomly divided into 7 groups,which including sham operation group,model control group,salvianolic acid capsule group(25,50,100 mg/kg),salvia tablets group(275 mg/kg),Diaoxinxuekang capsule group(100 mg/kg).The acute myocardial ischemia model in Beagle dogs were established by ligating anterior descending coronary artery after duodenal administration.The degree of myocardial ischemia was detected by epicardial electrogram mapping and the area of myocardial infarction was determined by NBT immunohistochemistry.The levels of serum CK and LDH were detected.The heart muscle ischemia protection of salvianolic acid capsule were observed.RESULTS: The salvianolic acid capsule could degrade the extent of heart muscle ischemia(∑-ST),decrease the range of heart muscle ischemia(N-ST),deflate the myocardial infarction size(MIS),relieve the levels of serum CK and LDH significantly,and relieve the injury degree of cadiocyte.CONCLUSION: Salvianolic acid capsule has remarkable protective effect on acute myocardial ischemia in Beagle dogs.更多还原