【摘要】 目的:探讨卡介苗体外对毛细支气管炎患儿免疫功能的影响。方法:取健康及毛细支气管炎患儿外周血体外分离单核细胞,加入不同浓度的卡介苗(BCG)培养,观察BCG对IL-12、IL-10水平的影响;同时分离外周血的淋巴细胞,加入经BCG处理的单核细胞培养液,用生理盐水作为阴性对照,培养满3d收集培养上清液,检测细胞因子IL-4水平。结果:体外实验结果显示BCG刺激单核细胞分泌的细胞因子水平(IL-10、IL-12)在一定浓度下呈浓度依赖性,BCG浓度为50μL/mL时,单核细胞分泌的细胞因子IL-10水平最高;IL-4的水平下降,与干预前相比具有统计学差异;IL-12升高不显著。结论:适宜浓度的BCG具有增强TH1的作用,IL-4水平降低推测可能与IL-10诱导产生调节性T细胞(CD4+CD25+Tr)有关。更多还原

【Abstract】 AIM:To approach the effects of Bacille Calmette-Guerin(BCG)on immunological function of infants with bronchiolitis in vitro. METHODS: The monocytes were segregated from the peripheral blood of infants with acute bronchiolitis and healthy infants as control. The cytokines of IL-10 and IL-12 were measured by ELISA after interfered with different concentrations of BCG in vitro. IL-4, produced by lymphocytes treated with the cultural supernatant of BCG-stimulated monocytes were determined by ELISA. Acute bronchiolitis infants and health children treated with normal saline were as control. RESULTS: BCG stimulated IL-10 and IL-12 production in a dose response manner in a certain concentration range. The higher level of IL-10 was observed when BCG concentration reached 50 μL/mL, while IL-12 had no significant increase. The level of IL-4 was markedly decreased compared with pre-treatment (P<0.05). CONCLUSION: Proper concentrations of BCG could induce a TH1 cytokine response effectively, the level of IL-4 was degraded after BCG intervention, presuming concerned with regular T cells(CD4+CD25+Tr)induced by IL-10.更多还原