【摘要】 目的:观察结缔组织生长因子(CTGF)在两肾一夹高血压大鼠肾间质中的表达及其与肾损害的关系,探讨罗格列酮对CTGF在肾间质表达的影响及高血压肾保护作用的机制。方法:两肾一夹高血压大鼠随机分为阳性对照组(B组)、罗格列酮治疗组(C组),假手术组作为阴性对照组(A组)。监测各组大鼠各阶段血压的变化,检测尿β2微球蛋白(β2-MG)、尿素氮(Bun)、血肌酐(Cr)。免疫组化法检测CTGF在肾组织中的表达,并判断肾组织的病理学变化及肾间质纤维化程度。结果:治疗前B组与C组的尿β2-MG均较A组明显升高(P<0.01),B组与C组之间的差异无统计学意义(P>0.05),而治疗后C组的尿β2-MG较B组明显降低(P<0.05);CTGF在B组和C组的表达较A组均明显增加(P<0.01),而C组CTGF的表达较B组降低(P<0.05)。CTGF的表达与β2-MG及肾间质纤维化呈正相关(r=0.734,P<0.01;r=0.777,P<0.01)。结论:罗格列酮可以降低血压,减少蛋白尿,下调CTGF在肾间质的表达,从而延缓肾间质纤维化(RIF)的发展,发挥肾保护作用。更多还原

【Abstract】 AIM:To observe the expression of connective tissue growth factor(CTGF) in renal interstitium of two-kidney one-clip hypertensive rats and the relationship between CTGF and renal damage, and to investigate the influnece of rosiglitazone on the expression of CTGF in renal interstitium and the mechanisms of renal protection of rosiglitation. METHODS: Two-kidney one-clip hypertensive rats were divided randomly into the positive control group(group B) and the rosiglitazone therapy group(group C),sham operated group were fed as the negative control group(group A). The changes of blood pressure were monitored in each stage of experiment of all groups. The urine β2-MG,blood urea nitrogen (Bun) and serum creatinine (Cr) were detected,the expression of CTGF in the nephridial tissue was detected by immunohistochemistry, the pathological changes and the degree of renal interstitial fibrosis respectively were judged. RESULTS: Compared with group A the contents of β2-MG were markedly increased in group B and C(P<0.01) before the treatment,there was no significant difference between group B and C (P>0.05). Compared with group B,the contents of β2-MG in group C were decreased significantly(P<0.05) after the treatment.The expressions of CTGF in group B and C were significantly increased compared with A (P<0.01), the expressions of CTGF were decreased in group C (P<0.05) compared with group B. There was a significant positive correlation between the expressions of CTGF,the contents of β2-MG and the scores of renal interstitium fibrosis (r=0.734, P<0.01; r=0.777, P<0.01). CONCLUSION:Rosiglitazone can delay the development of renal interstitial fibrosis by reducing blood pressure and albuminuria,and downregulating the expressions of CTGF in renal interstism so it educes kidney protection.更多还原