【摘要】 对生防链霉菌Ⅲ-61产生抗真菌活性物质的摇瓶发酵工艺进行了研究。利用正交试验设计优化了发酵培养基组分,其最适配方为黄豆粉1.5%,蛋白胨0.3%,蔗糖1.0%,淀粉1.3%,磷酸二氢钾0.02%,硫酸镁0.025%,氯化钠0.5%,配成水溶液,调pH至7～7.4,加碳酸钙1%。通过单因素试验,筛选获得了最优培养条件组合:液体种龄24h,接种量5%～10%,500mL摇瓶培养基装量为80mL,摇床转速240r/min,培养温度31℃,发酵周期96～120h。此优化的发酵培养基与发酵条件的组合所得菌株Ⅲ-61发酵液对主要靶标黄瓜灰霉病菌的抑菌圈直径达49.5mm,较优化前提高了45.59%。更多还原

【Abstract】 A study on the fermentation process in shake flask to produce antifungal active substance by Streptomyces Ⅲ-61,a biocontrol strain isolated from the forest soil in a suburb of Beijing,was launched.The fermentation medium was optimized by orthogonal experiment.The result showed that the optimal liquid medium was composed of 1.5% soybean meal,0.3% peptone,1.0% sucrose,1.3% soluble starch,0.02% KH2PO4,0.025% MgSO4·7H2O and 0.5% NaCl.The initial pH of the solution was adjusted to 7.0—7.4,then 1% CaCO3 was added.The culture conditions were screened with mono-factor test,and the most suitable technical parameters were summed up as follows:the liquid seed was cultured for 24 hours,the fermentation was carried out in 500mL erlenmeyer flask with 80mL production medium inoculated with 5%—10% seed culture and incubated at 240 r/min,31℃ for 96—120 hours.The inhibition zone diameter against Botrytis cinerea up to 49.5mm by the germfree filtrate from fermented broth of strain Ⅲ-61 obtained with the optimized fermentation process,which is 45.59% higher than that produced by the basic medium and initial fermentation process.更多还原