【摘要】 目的研究精脒(SPD)及多胺合成抑制剂α-双氟甲基鸟氨酸(DFMO)对PC12细胞生长特性的影响。方法取对数生长期嗜铬细胞瘤细胞株PC12细胞,分为SPD组和DFMO组分别培养4 d,每天观察PC12细胞生长情况。光学显微镜下观察细胞形态学变化;台盼蓝染色法绘制细胞生长曲线。用四氮唑盐(MTT)比色试验检测细胞的生长或抑制,流式细胞仪检测细胞周期分布。结果SPD作用PC12细胞后细胞数较对照组明显增多,胞体更加粗大,细胞突起更加细长,分支相连。DFMO作用PC12细胞后大部分细胞变圆,细胞突起变短。细胞生长曲线显示SPD组活细胞数明显增多,DFMO组细胞数减少。SPD作用4 d对细胞生长有促进作用,DFMO作用4 d对细胞生长有明显抑制作用。6 mmol/L DFMO作用4 d可使PC12细胞生长周期阻滞在S期。结论SPD在一定浓度范围内对PC12细胞生长具有促进作用,DFMO在一定浓度范围内对PC12细胞则具有抑制作用。更多还原

【Abstract】 Objective To study the effects of spermidine(SPD) and polyamine synthesis inhibitor,α-difluoromethyl ornithine(DFMO),on the growth of pheochromocytoma(PC12) cells.Methods PC12 cells in logarithm period were divided into group SPD and group DFMO.PC12 cells were observed every day for 4 days.The morphological changes of PC12 cells were investigated with light microscope.Cell growth curve was made with trpanblau staining.The cell viability was examed by MTT.The cell cycle was monitored with flow cytometry.Results The cell bodies were morphologically bigger and the length of neurite longer after exposed to SPD for 4 days than those in control group.The length of the neurite was shorter after exposed to DFMO for 4 days than that in control group.The cell growth curve showed that the cell growth was promoted by SPD,but inhibited by DFMO.SPD of different doses promoted,but DFMO suppressed the proliferation of PC12 cells after exposure for 4 days.The cell cycle of the PC12 cells could be inhibited in S period at a concentration of 6 mmol/L DFMO on the 4th day.Conclusion The results suggest that the growth and proliferation of PC12 cells can be promoted by SPD,but suppressed,by DFMO at a certain concentration.更多还原