【摘要】 目的通过研究机械压应力作用下炎症因子对滑膜细胞MMP-2,-9活性的影响,探讨滑膜组织在前交叉韧带(anterior cruciate ligament,ACL)机械性损伤过程中所起的作用。方法利用等双轴拉伸装置对滑膜细胞施加12%模拟机械损伤的压应力以及施加炎症因子IL-1α与TNF-α,通过明胶酶谱法检测MMP-2,-9的活性。结果12%机械压应力使MMP-2的活性增加了122%,TNF-α对MMP-2的活性有着明显的时间依赖性增加,而IL-1α则没有;IL-1α与TNF-α共同作用则对MMP-2,-9活性有着显著增加。结论机械压应力与炎症因子对MMP-2,-9的活性有着显著的协同作用,机械压应力与炎症因子能够增加滑膜细胞MMP-2,-9的活性,因此滑膜组织可能参与调节关节腔内微环境以及膝关节组织损伤与修复的过程。更多还原

【Abstract】 Objective To explore the role of synovium in the process of anterior cruciate ligament (ACL) injury by studying the effects of inflammatory on the activities of MMP-2,-9 under mechanical compression in human synoviocytes. Method We used an equi-biaxial stretch chamber with 12% mechanical injury compression and inflammatory factors (IL-1α and TNF-α) in human synoviocytes, and detected the activities of MMP-2,-9 under different conditions by zymography. Result Mechanical compression increased the MMP-2 production by 122% under 12% compression. In addition, TNF-α can also elevate the activity of MMP-2 in a time dependent way, while IL-1α cannot. However, mixture of these two factors dramatically increased MMP-2,-9 production. Mechanical injury had a strong synergistic effect on MMP-2,-9 productions with TNF-α and IL-1α. Conclusions Mechanical and inflammatory factors can elevate the activities of MMP-2,-9. Thus, synvoium might involve in regulating the micro-environment of joint cavity and the injury/healing process of knee joint tissue.更多还原