【摘要】 目的:探讨DMSO对运动诱导的大鼠海马神经发生的影响。方法:将30只雄性5周龄SD大鼠随机分为对照组、运动组、DMSO+运动组,通过预先埋管方式,向DMSO+运动组动物侧脑室内注射DMSO,注射1小时后,运动组和DMSO+运动组动物进行跑台运动30min,运动方案为:5m/min,5min;8m/min,5min;11m/min,20min。1周后所有动物取材,采用免疫荧光染色技术和荧光显微镜,检测各组大鼠海马齿状回内新生细胞的数量。结果:各组动物每个脑切片海马齿状回内BrdU免疫阳性细胞数平均为:对照组89.92±12.77个,运动组119.19±19.94个,DMSO+运动组73.94±17.36个。DMSO+运动组的BrdU+细胞数量较运动组减少了37.96%,BrdU++NeuN+细胞数量减少了40.56%。结论:DMSO对运动诱导的大鼠海马神经发生有显著的抑制作用。更多还原

【Abstract】 Objective To investigate the effect of DMSO(dimethyl sulfoxide) on the exercise-induced hippocampal neurogenesis in rats.Methods Thirty male SD rats of 5 weeks old were randomly divided into 3 groups:control group(C),exercise group(E)and DMSO plus exercise group(DE).One hour after injection of DMSO through cannula(planted into lateral cerebral ventricle before exercise),all rats except group C were forced to run on treadmill for 30 min per day,the running intensity increased gradually:5m/min for 5min,8m/min for 5min and 11m/min for 20min.A week later,all the rats were sacrificed,immunofluorescence staining was used to evaluate the level of neurogenesis.Results The number of BrdU positive cells in dentate gyrus of hippocampus was:89.92±12.77 in group C,119.19±19.94 in group E,and 73.94±17.36 in group DE,respectively.As comparing with that in group E,the number of BrdU positive cells in group DE was decreased by 37.96%,the number of BrdU+NeuN positive cells decreased by 40.56%.Conclusions DMSO significantly inhibits exercise-induced neurogenesis in hippocampus of rats.更多还原