【摘要】 目的探讨野生云芝多糖水溶性新组分CVPS-B对RAW264.7巨噬细胞膜硫酸软骨素-蛋白聚糖(CS-PGs)结合氧化修饰低密度脂蛋白(Ox-LDL)的影响。方法分别用溴化氰活化的Sepharose CL-4B亲和层析及体外结合CS-A的方法分别测定CS/PGs与Ox-LDL的结合率;用离子交换层析提取RAW264.7巨噬细胞膜PGs;用1,9-二甲基亚甲基蓝(DMMB)分光光度法测定糖胺聚糖。结果在17μmol MDA·g-1protein Ox-LDL水平,不同剂量CVPS-B(10、50及100μg)在体外降低Ox-LDL(200μg)与CS-A(100μg)结合,分别降低14%、29%(P<0.05)及43%(P<0.01)。不同剂量CVPS-B(10、50及100μg)在体外降低Ox-LDL(17μmol MDA·g-1 protein)与巨噬细胞膜表面PGs结合,分别降低8%、27%(P<0.05)及38%(P<0.01)。不同剂量CVPS-B可抑制Ox-LDL(50mg·L-1,17μmol MDA·g-1 protein)诱导泡沫细胞的形成。结论CVPS-B可体外抑制巨噬细胞膜CS-PGs结合Ox-LDL。更多还原

【Abstract】 Aim To identify the effects of CVPS-B on RAW264.7 macrophage plasma membrane chondroitin sulfate (CS)-proteoglycans (PGs) binging oxidized low-density lipoprotein (Ox-LDL).Methods Rates of PGs binding Ox-LDL were analyzed by their abilities to bind Ox-LDL coupled to a CnBr-activated Sepharose CL-4B chromatography,and rates of CS-A binding Ox-LDL were determined in vitro.RAW264.7 macrophages membrane PGs were isolated by anion exchange chromatography.Glycosaminoglycans were analyzed through the DMMB spectrophoto-metric assay.Results At the level of LDL oxidation (17 μmol MDA·g-1 Pro),however,CVPS-B (10,50,100 μg) in vitro decreased the ability of CS-A (100 μg ) binding Ox-LDL (200 μg) by 14%,29% (P<0.05),and 43% (P<0.01),respectively. Furthermore,the binding of the PGs to Ox-LDL (17 μmol MDA·g-1 Pro) was decreased by 8%,27% (P<0.05),and 38% (P<0.01),respectively after CVPS-B (10,50,and 100 μg) being pre-incubated. CVPS-B could inhibit Ox-LDL (50 mg·L-1,17 μmol MDA·g-1 Pro)-induced foam cell formation.Conclusions These data provide the first evidence that CVPS-B can inhibit the macrophage surface CS-PGs binding Ox-LDL in vitro.更多还原