【摘要】 目的探讨实时荧光PCR技术检测男性性病尿道炎及男性尿道炎后综合征(MPUS)患者的病原体的临床价值。方法用实时荧光PCR法对718例男性性病尿道炎及MPUS患者尿道分泌物或前列腺液同时作淋球菌(NG)、沙眼衣原体(C t)、解脲支原体(UU)检测。结果52.2%的淋病患者并发C t、UU感染。非淋菌性尿道炎(NGU)患者C t、UU阳性率68.2%,C t阳性率31.1%、UU阳性率42.6%,5.4%患者C t、UU合并感染。MPUS患者NG、C t、UU阳性率仅为10.2%。结论实时荧光PCR技术灵敏度高,特异性强,对男性性病尿道炎及MPUS的诊断和治疗具有重要的指导意义。更多还原

【Abstract】 Objective To discuss the clinical effectiveness of real-time PCR in detecting the pathogens of male′s sexually transmitted urethritis and male′s post-urethritis syndrome(MPUS).Methods Real-time PCR was used to detect the pathogens including Neisseria gonorrhoeae(NG),Chlamydia trachomatis(Ct) and Ureaplasma urealyticum(UU) in the urinary secretions or succus prostaticus from 718 male patients with sexually transmitted urethritis and/or post-urethritis sydrome.Results The prevalence of Ct and UU was 52.2% in the gonorrhoea patients and was 68.2% in the patients with nongonococcal urethritis(NGU).The positive rate was 31.1% for Ct and 42.6% for UU in NGU and the co-infection rate of Ct and UU was 5.4%,while the total prevalence of Ct,NG and UU was only 10.2% in the MPUS patients.Conclusion The technique of real-time PCR has very high sensitivity and specificity.It serves as significant guidance in the diagnosis and treatment of male′s sexually transmitted urethritis and MPUS.更多还原