【摘要】 目的研究盐酸戊乙奎醚(penehyclid ine hydrochloride,PHC)对大鼠缺氧缺糖/复氧复糖海马脑片的保护作用及机制。方法采用大鼠海马脑片缺氧缺糖/复氧复糖模型;分为正常组、缺氧缺糖/复氧复糖(H/R)组、PHC2μmol/L(PHC2组)、PHC 10μmol/L(PHC10组)、PHC 50μmol/L(PHC50组)、Sco 50μmol/L(Sco50组),测量各组LDH释放率,免疫组织化学法测定各组海马CA1区Bax、Bcl-2、Caspase-3的表达。结果PHC各组LDH释放率均较H/R组下降,以PHC50组效果最好(P<0.01)。PHC50组Bcl-2阳性细胞的表达和Bcl-2/Bax比值均较H/R组升高,Bax和Caspase-3阳性细胞表达均较H/R组降低(P(0.01)。Sco50组Bcl-2阳性细胞的表达和Bcl-2/Bax比值也较H/R组升高,Bax和Caspase-3阳性细胞表达均较H/R组降低(P(0.01)。结论PHC可能通过减少LDH释放和Caspase-3、Bax的表达,增加Bcl-2的表达减轻大鼠缺氧缺糖/复氧复糖海马脑片的损伤。更多还原

【Abstract】 Objective To investigate the protective effect of penehyclidine hydrochloride(PHC) on the apoptosis of rat hippocampal slices induced by hypoxia/hypoglycemia and reoxygenation(H/R) and the underlying mechanism.Methods Experimental model of hypoxia/hypoglycemia and reoxygenation/glucose reintroduction was established by adoption of rat hippocampal slices,which were equally divided into 6 groups: Control group,H/R group,PHC 2 μmol·L-1(PHC2) group,PHC 10 μmol/L(PHC10) group,PHC 50 μmol/L(PHC50) group and Sco 50 μmol/L(Sco50) group.The LDH release rate was used to evaluate the protective effect of PHC on the apoptosis of rat hippocampal slices induced by H/R.The expression of Bax,Bcl-2 and Caspase-3 in hippocampal CA1 field of each group were respectively detected by immunohistochemical method.Results Compared with H/R group,the LDH release rate in PHC groups decreased,with PHC50 group on the top;the expression of Bcl-2 positive cells and the Bcl-2/Bax ratio in PHC50 group increased;and the expression of Bax and Cspae-3 positive cells decreased(P<0.01).Compared with H/R group,the expression of Bcl-2 positive cells and the Bcl-2/Bax ratio in Sco50 group increased;and the expression of Bax and Cspae-3 positive cells decreased(P<0.01).Conclusion PHC might attenuate the H/R-induced injuries to hippocampal slices by reducing the release rate of LDH,the expression of Caspase-3 as well as Bax and increase the expression of Bcl-2.更多还原