【摘要】 目的探讨在1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)致小鼠黑质多巴胺(DA)能细胞发生凋亡时,calb ind in-D-28k(CB)抗细胞凋亡的作用机制。方法MPTP连续5天腹腔注射构建中脑黑质DA能细胞损伤的小鼠模型,模型鼠脑黑质致密部立体定位注射携带CB基因的高滴度慢病毒颗粒(CB-H IV-Ⅰ),W estern b lotting方法检测CB的在体过表达以及鼠脑黑质部位凋亡相关蛋白Bax的表达变化。结果与空白对照组(control)和黑质定位注射空病毒颗粒组(H IV-Ⅰ)相比,注射CB-H IV-Ⅰ的实验组的黑质中CB的表达量显著升高(P<0.05),而Bax的表达量明显降低(P<0.05)。结论病毒颗粒CB-H IV-Ⅰ携带的CB基因在黑质细胞中获得了高效的表达;凋亡相关蛋白Bax可能参与了CB对黑质DA能神经细胞的保护作用。更多还原

【Abstract】 Objective To investigate the anti-apoptotic mechanism of calbindin-D-28k(CB) in the MPTP-induced apoptosis of dopaminergic(DA) neurons in midbrain substantia nigra in mice.Methods MPTP was injected into the peritoneal cavity in 5 consecutive days to establish an injury model of DA neurons in the midbrain substantia nigra in mice,and high titer lentivirus particles carrying CB(CB-HIV-Ⅰ) were injected into the midbrain substantia nigra by stereotaxic apparatus.Western blotting was used to analyze the expression levels of CB and apoptosis-related protein Bax in substantia nigra,respectively.Results The expression level of CB increased(P<0.05),while that of Bax decreased in CB-HIV-Ⅰgroups compared with control groups and HIV-Ⅰgroups(P<0.05).Conclusion The lentivirus particles of CB-HIV-Ⅰwere highly expressed in substantia nigra cells;apoptosis-related protein Bax might participate in the protective effect of CB on DA neurons in substantia nigra.更多还原