【摘要】 目的探讨急性和重复性心理应激对大鼠某些细胞免疫功能的影响。方法将60只Sprague-dawley(SD)大鼠随机分为6组,每组10只,3个应激组分别接受为期1、14和28 d束缚应激(6 h/d),相应的3个对照组不接受任何应激处理。分别测定大鼠在应激和非应激状态下脾自然杀伤(NK)细胞活力和T淋巴细胞增殖活力。结果反复应激束缚14和28 d组大鼠的脾质量[14 d:(623.75±62.10)mg vs(985.98±127.79)mg,28 d:(672.50±49.82)mg vs(1 055.75±94.96)mg]、脾指数[14 d:(0.20±0.01)vs(0.27±0.02),28 d:(0.19±0.01)vs(0.27±0.02)]、胸腺质量[14 d:(319.88±76.30)mg vs(512.25±106.06)mg,28 d:(312.75±48.00)mg vs(488.13±34.56)mg]及胸腺指数[14d:(0.10±0.02)vs(0.15±0.03),28 d:(0.09±0.01)vs(0.13±0.02)]均低于相应的对照组大鼠,同时,反复应激组大鼠的脾脏T淋巴细胞增殖功能[(14 d时ConA5 mg/L:(57 587.75±9 958.97)cpm vs(154 919.88±4 961.22)cpm,14 d时ConA2.5 mg/L:(52 323.50±530.71)cpm vs(102 047.63±3 329.69)cpm;28 d时ConA5.0 mg/L:(52 350.00±2 500.89)cpm vs(153 460.88±11 971.49)cpm,28 d时ConA2.5 mg/L:(42 322.38±403.85)cpm vs(100 068.63±11 326.51)cpm]及脾脏NK细胞的活力也低于相应的对照组大鼠;而急性应激束缚1 d组大鼠的各项指标与对照组相比无明显差异。结论反复应激刺激对大鼠的某些细胞免疫功能具有抑制效应。更多还原

【Abstract】 Objective To explore the effect of acute and repeated psychological stress on certain cellular immune function in rats.Methods 60 SD rats were randomly divided into 6 groups,each consisting of 10 rats.The rats in three stress groups were subjected to either a single day or 14,28 days of restraint(6 h/d),and those in the corresponding control groups were transferred to the testing room without any treatment.Spleen natural killer cell activity and T-lymphocyte proliferation of acutely stressed and unstressed rats were measured.Results The results showed that the spleen weight[14 d:(623.75±62.10)mg vs(985.98±127.79)mg,28 d:(672.50±49.82)mg vs(1 055.75±94.96)mg],spleen index[14 d:(0.20±0.01) vs(0.27±0.02),28 d:(0.19±0.01) vs(0.27±0.02)],thymus gland weight[14 d:(319.88±76.30)mg vs(512.25±106.06)mg,28 d:(312.75±48.00)mg vs(488.13±34.56)mg]and thymus gland index[14 d:(0.10±0.02) vs(0.15±0.03),28 d:(0.09±0.01) vs(0.13±0.02)]in the repeated stress group were lower than those of their corresponding control groups.The results also showed that repeated stress could cause a significant impairments of T-lymphocyte proliferation[ConA 5.0 mg/L for 14 d:(57 587.75±9 958.97) cpm vs(154 919.88±4 961.22) cpm,ConA 2.5 mg/L for 14 d:(52 323.50±530.71) cpm vs(102 047.63±3 329.69) cpm;ConA 5 mg/L for 28 d:(52 350.00±2 500.89) cpm vs(153 460.88±11 971.49) cpm,ConA 2.5 mg/L for 28 d:(42 322.38±403.85) cpm vs(100 068.63±11 326.51)cpm]and spleen natural killer cell activities when compared with their corresponding control groups.Conclusion Repeated psychological stress could be associated with the cellular immune suppression.更多还原