【摘要】 本研究旨在建立骨髓涂片间期荧光原位杂交(I-FISH)的实验方法,为检测多发性骨髓瘤(MM)分子细胞遗传学提供新方法。以骨髓涂片为载体,通过一系列的处理,固定及消化后,用8号染色体着丝粒探针行I-FISH分子细胞遗传学的检测,并比较该方法与常规I-FISH结果的差异。结果表明:两种方法分析非恶性血液病标本的各个信号比例无统计学差异(p>0.05)。骨髓涂片I-FISH研究中,19例MM患者中8例(42.1%)有8号染色体异常,其中8号染色体单体(-8)5例(26.3%),8号染色体三倍体(+8)3例(15.8%)。结论:骨髓涂片I-FISH具有操作简便、经济、准确的特点,可用于MM分子遗传学异常的研究。更多还原

【Abstract】 This study was aimed to establish the technique of interphase fluorescence in situ hybridization(I-FISH) used on smear of bone marrow directly,and to develop a new method for detection of the molecular cytogenetics in multiple myeloma(MM).After a series of treatment,fixation and digestion of the bone marrow smear as the carrier,the chromosome 8 centromere probe were used in I-FISH for molecular cytogenetics detection.At the same time,differences were compared in the results between the new method and the conventional I-FISH.The results showed that there was no statistically significant difference of proportion of various signals in non-hematologic malignancies when detecteding with the two methods(p>0.05).In bone marrow smear I-FISH,8 out of 19 cases(42.1%) had abnormality of chromosome 8,including 5 cases with-8(26.3%) and 3 cases with +8(15.8%).It is concluded that the I-FISH on smear of bone marrow is characterized by convenience,economy and accuracy.Therefore,it can be used for research of molecular cytogenetics in MM.更多还原