【摘要】 本研究利用脐血CD34+细胞在体外大量扩增红系细胞为解决血源短缺、杜绝输血传染、克服稀有血型患者疑难配血等问题提供一种有益的途径。利用添加SCF、IL-3、EPO等细胞因子的无血清培养液扩增脐血来源的CD34+细胞,并通过间充质干细胞的支持培养来诱导其向红系细胞扩增和分化。结果表明,经过23天培养以后,本方法可以使细胞的扩增倍数达到2.52×105,其中95%以上都是红系细胞,粒单系细胞和巨核细胞所占比例都在1%以内。无间充质干细胞的培养体系在细胞扩增数量及红系细胞所占比例方面都明显逊于有间充质干细胞支持的培养体系。结论:利用细胞因子和间充质干细胞可以在体外大量扩增红系细胞,其中间充质干细胞对红系细胞增殖和分化起重要的促进作用。更多还原

【Abstract】 This study was aimed to investigate a beneficial approach for resolving the deficiency of blood source,preventing the infection resulting from blood transfusion and overcoming the knotty match of patients with rare blood group by using massive expansion of erythroid cells from cord blood CD34+ cells in vitro. The CD34+ cells from human cord blood were cultured in serum-free medium supplemented with stem cell factor (SCF),interleukin-3 (IL-3) and erythropoietin (EPO) for 1 week,then expansion and differentiation of CD34+ cells into erythroid cells were supported by co-culture with human mesenchymal stem cells (MSCs) derived from bone marrow for 2 weeks. The results indicated that after culture for 23 days,the expansion multiple of total cell number reached 2.52×105,and over 95% of these cells were erythroid cells as compared with less than 1% of myelomonocytic (CD14+ or CD15+) cells and megakaryocytic(CD41+) cells. However,the culture system without MSC support was significantly disadvantaged both in expansion ability and ratio of erythroid cells when compared with MSC supporting system. It is concluded that the erythroid cells can be produced from CD34+ cells in large scale by culturing in the system comprised of cytokine sets and MSC feeders,in which MSCs can support the proliferation and differentiation of erythroid cells.更多还原