【摘要】 本研究旨在探讨藤黄酸对高危组骨髓增生异常综合征(MDS)细胞的生长抑制作用及其机制。以人MDS-RAEB细胞株MUTZ-1细胞为研究对象,采用MTT法测定增殖抑制率,流式细胞术检测细胞凋亡及细胞周期变化,用RT-PCR检测bax/bcl-2基因表达。结果表明,藤黄酸对MUTZ-1细胞有生长抑制作用,在0.2-0.8μg/ml浓度范围内随着药物浓度的增加MUTZ-1细胞的增殖抑制率增高;流式细胞学检测发现,藤黄酸浓度0、0.4、0.6、0.8μg/ml引起的MUTZ-1凋亡率分别为(5±0.5)%、(13±0.5)%、(37±0.7)%和(56±0.6)%,而且凋亡率随着药物浓度增加而增加(p<0.05)。bcl-2基因表达程度随藤黄酸剂量增加而减弱,而baxmRNA表达无明显变化。结论:藤黄酸通过诱导MUTZ-1细胞凋亡、下调bcl-2基因表达而抑制该细胞生长,这可能是藤黄酸抗肿瘤的主要机制之一。更多还原

【Abstract】 This study was aimed to investigate the effects of gambogic acid on the cells of high-risk patients with myelodysplastic syndrome(MDS)in vitro and its mechanism.The inhibition effect of gambogic acid on growth of MUTZ-1 cell line of MDS-RAEB was detected by MTT method.Apoptosis and cell cycle were detected by morphological observation and flow cytometry respectively.The expressions of bax/bcl-2 gene at mRNA level were detected by RT-PCR.The results indicated that the Gambogic acid inhibited the growth of MUTZ-1 cells,the inhibitory rate of gambogic acid with the range of 0.2-0.8 μg/ml was enhanced along with increasing of drug concentration.Flow cytometric assay showed that the apoptotic rate of MUTZ-1 cells treated by gambogic acid also was enhanced along with increasing of drug concentration,the apoptotic rates resulting from gambogic acid(0,0.4,0.6,0.8 μg/ml)were(5±0.5)%、(13±0.5)%、(37±0.7)% and(56±0.6)% respectively.The characteristic changes of apoptosis emerged in MUTZ-1 cells after being exposed to gambogic acid.Gambogic acid could significantly down-regulate the expressions of bcl-2 gene in a dose dependent manner,however,it had no effects on bax gene.It is concluded that within the range of concentration from 0.4 to 0.8 μg/ml,gambogic acid can inhibit the growth of MUTZ-1 cells by inducing their apoptosis and down-regulating the expression of bcl-2 gene,which may be one of the main mechanisms underlying its antitumor effects.更多还原