【摘要】 本研究探讨As2O3对肿瘤细胞中COX-2、MMP-2和MMP-9表达的影响。以体外培养SGC7901和K562细胞为对象,加不同浓度As2O3干预40和72小时。采用Western blot法和荧光定量RT-PCR法分别测定COX-2、MMP-2和MMP-9的表达和SGC7901细胞内的COX-2和MMP-2 mRNA的水平。结果显示:As2O3干预后SGC7901和K562细胞内的COX-2、MMP-2和MMP-9的表达水平呈时间和剂量依赖性减低;MMP-2 mRNA和COX-2 mRNA减低。结论:三氧化二砷可抑制肿瘤细胞COX-2、MMP-2和MMP-9表达,这一机制可能参与砷剂抑制肿瘤血管生成的过程。更多还原

【Abstract】 This study was aimed to investigate on effect of As2O3 on expressions of COX-2,MMP-2 and MMP-9 in SGC7901 and K562 cells.SGC7901 and K562 cells were cultured in RPMI 1640 medium and were inoculated in culture medium with different concentrations of As2O3 and at different times.Expressions of COX-2,MMP-2 and MMP-9 in SGC7901 and K562 cells were measured by using Western blot,while the levels of COX-2 mRNA and MMP-2 mRNA were measured with fluorescence quantitative RT-PCR.The results showed that the expression of COX-2,MMP-2 and MMP-9 decreased in dose-and time-dependent manners after treating with As2O3.The levels of COX-2 mRNA and MMP-2 mRNA reduced in groups treated with As2O3.In conclusion,As2O3 inhibits expressions of COX-2,MMP-2 and MMP-9 in K562 and SGC7901 cells,suggesting that As2O3 inhibits tumor development throung its effect on angiogenesis involved in solid and hematologic malignancies.更多还原