【摘要】 建立采用高效液相色谱法测定5-FPE脂质体包封率的方法。色谱柱为Kromasil 100 A C18column(350mm×4.6mm,5μm),流动相为甲醇-水(58:42,v/v),流速为0.8mL/min,测定波长为271nm,进样量为10μL,柱温为30oC。线性范围为0.8～12.8μg/mL,相关系数r=0.9999,日内和日间精密度的RSD小于2.97%,平均回收率为96.8%～104.6%,RSD小于2.24%。本文建立了采用高效液相色谱测定5-FPE脂质体包封率的方法,该方法简便、快速、精确、灵敏度高。更多还原

【Abstract】 A simple HPLC method was established for the determination of entrapment efficiency of a new 5-FPE liposome formulation.Chromatographic separation was performed on a Kromasil 100 A C18 column(350 mm×4.6 mm,5μm).The mobile phase was consisted of methanol-water(58:42,v/v).The flow rate of mobile phase was set at 0.8 mL/min.The UV detection wavelength was 271 nm,and the column temperature was 30 oC.The linear range of 5-FPE was from 0.8-12.8 μg/mL,r= 0.9999.The RSD of intra-day and inter-day precision were less than 2.97%.The average recovery was from 96.8%-104.6% with RSD less than 2.24%.The method was simple,rapid,accurate,and sensitive.It is suitable for the determination of entrapment efficiency of the 5-FPE liposome formulation.更多还原