【摘要】 目的:研究犬急性心房颤动(房颤)时心房内皮细胞环氧酶-2(COX-2)的变化。方法:12条健康成年杂种犬被随机分为快速心房起搏(RAP)组(6只)与正常对照组(6只),PAP组建立犬急性房颤动物模型。分别取两组犬的左心耳、右心耳部位心肌组织,应用免疫组织化学技术检测心耳内皮细胞COX-2的改变。结果:(1)RAP组所有犬均可诱发出阵发性房颤;(2)RAP组犬心耳内皮细胞COX-2阳性率为83.3%(10/12),对照组为25.0%(3/12),RAP组显著高于对照组(P=0.013)。结论:急性房颤时心耳内皮细胞COX-2水平增加,提示在房颤早期发生了炎症反应。更多还原

【Abstract】 Objective:To investigate the expression of cyclooxygenase-2(COX-2) in right and left atrial appendages in acute atrial fibrillation(AF) model of caine.Methods:Twelve adult mongrel canines were randomly divided into rapid atrial pacing(RAP) group(n=6) and sham control group(control group,n=6).A model of acute AF in RAP group has been developed.Immunohistochemistry was performed to detect the change of COX-2 at the protein level in endothelium of atrial appendages.Results:(1) Paroxysmal AF was induced in all of the canines of RAP group.(2) Immunohistochemistry study indicated that in RAP and control groups,the rate of COX-2 expression was 83.3%(1012) and 25.0%(312) respectively.COX-2 expression was significantly stronger in the former(P=0.013).Conclusion:Acute AF in canines led to COX-2 protein expressed in RAP group showed its inflammation mechanism.更多还原