【摘要】 目的评价快速诊断试纸条(DDIA)试剂盒半定量检测方法在血吸虫病流行病学调查中应用的可能性。方法在3个不同流行程度的血吸虫病流行区分别随机选择300、280例和230例当地居民作为检测对象,同时进行粪便病原学检查和用DDIA试剂盒进行抗体水平的半定量检测,通过相关分析评价人群抗体水平和感染率之间的关系。结果人群血吸虫抗体阳性率和抗体几何平均滴度(GRMT)均与粪检阳性率呈直线正相关关系,直线回归方程分别为YI=0.538X-12.470和YG=26.170X-28.761,r分别为0.9579和0.9924(P均<0.05)。结论DDIA试剂盒可以用于血吸虫病流行区人群抗体水平的半定量检测,并以人群GRMT采用直线回归方程(YG=26.170X-28.761)推算出人群感染率的理论值。更多还原

【Abstract】 Objective To evaluate the possibility of application of semiquantitative test of dipstick dye immunoassay(DDIA) kit in epidemiological survey in the schistosome endemic areas.Methods The local residents,300,280 and 230 persons,respectively,in three endemic regions with different prevalences were served as sampling,and detected with the semiquantitative test of DDIA kit and parasitological examination(stool hatching),and then the relationship between the anti-schistosome antibody level and the prevalence(positive stool examination) was analyzed.Results Both the antibody positive rate and geometric reciprocal mean titer(GRMT) showed positive correlations with the prevalence(the regression equations were YI=0.538X-12.470 and YG=26.170X-28.761,r(s) were 0.957 9 and 0.992 4,and P(s)<0.05 and 0.01,respectively).Conclusion The semiquantitative test of DDIA kit for schistosomiasis could be used for evaluation of the prevalence in the endemic regions.更多还原