【摘要】 目的分析肝素钠抗凝的血浆肌酸激酶、肌酸激酶同工酶MB、乳酸脱氢酶、α-羟丁酸脱氢酶、天门冬氨酸氨基转移酶的活性,探讨肝素钠对心肌酶活力的影响。方法收集经体检证实健康个体113例,其中男性61例,女性52例,同时采集他们的肝素钠抗凝血浆和血清。通过连续监测法测定血浆和血清肌酸激酶、肌酸激酶同工酶MB、乳酸脱氢酶、α-羟丁酸脱氢酶、天门冬氨酸氨基转移酶的活性。结果肝素钠抗凝血浆的肌酸激酶、肌酸激酶同工酶MB、乳酸脱氢酶、α-羟丁酸脱氢酶、天门冬氨酸氨基转移酶的活性均值分别为95.4±50.3u/L、13.1±8.1u/L、184.0±41.9u/L、122.7±32.9u/L、26.3±17.9u/L,而血清对照组的肌酸激酶、肌酸激酶同工酶MB、乳酸脱氢酶、α-羟丁酸脱氢酶、天门冬氨酸氨基转移酶的活性均值分别为95.9±51.4u/L、12.7±7.5u/L、187.1±36.3u/L、119.6±29.2u/L、26.5±18.5u/L,两种标本的心肌酶活性无显著性差异(所有P值均大于0.05)。结论肝素钠对心肌酶活性无显著影响,肝素钠抗凝血浆可作为心肌酶活性测定的标本,可为临床抢救急性心肌梗死赢得宝贵时间。更多还原

【Abstract】 Objective This study aimed to analyse the activity of creatine phospokinase,creatine phospokinase isoenzyme MB,lactic dehydrogenase,α-hydroxybutyrate dehydrogenase,and aspartate aminotransferase in plasma anti-coagulated by heparin sodium,and to explore the effect of heparin sodium on myocardial enzyme activity.Methods Peripheral blood samples were obtained from 113 healthy individuals(61 males and 52 females).Plasma anti-coagulated by heparin sodium and serum were separated from peripheral blood.The enzyme activity was determined by continuous monitoring assay for creatine phospokinase,creatine phospokinase isoenzyme MB,lactic dehydrogenase,α-hydroxybutyrate dehydrogenase,and aspartate aminotransferase.Results The enzyme activity of plasma anti-coagulated by heparin sodium were 95.4±50.3 u/L,13.1±8.1 u/L,184.0±41.9 u/L,122.7±32.9 u/L,and 26.3±17.9 u/L for creatine phospokinase,creatine phospokinase isoenzyme MB,lactic dehydrogenase,α-hydroxybutyrate dehydrogenase,and aspartate aminotransferase,respectively.While the enzyme activity of serum were 95.9±51.4 u/L,12.7±7.5 u/L,187.1±36.3 u/L,119.6±29.2 u/L,and 26.5±18.5 u/L for creatine phospokinase,creatine phospokinase isoenzyme MB,lactic dehydrogenase,α-hydroxybutyrate dehydrogenase,and aspartate aminotransferase,respectively.There was no significant difference between plasma anti-coagulated by heparin sodium and serum(all P > 0.05).Conclusions Heparin sodium has no effect on the activity of myocardial enzyme.Plasma anti-coagulated by heparin sodium can be used when analyzing the activity of myocardial enzyme.This will shorten test time for patients with acute myocardial infarction.更多还原