【摘要】 为寻找新型的、高活性的胰蛋白酶抑制剂,将紫花芸豆磨碎乙醚脱脂后制成花豆粉,用pH4.0的稀酸溶液抽提,并在70℃对抽提液热变性15min,然后进行40%～70%饱和度的硫酸铵分级沉淀。对花豆胰蛋白酶抑制剂的粗提条件进行了优化,并探索了不同温度处理对PVTI抑制活性的影响。结果表明:100℃处理60min后,活性丧失约30%,PVTI具有良好的热稳定性。更多还原

【Abstract】 Experiment was carried out to find new-style and high activity trypsin inhibitor and the extract conditions were optimized.The crude extracts from Phaseolus vulgaris was obtained by defating with ethyl ether,extracting with a pH 4.0 buffer of dilute acid,heating at 70 ℃ for 15min,and fractional precipitating with 40 %-70 % of (NH4)2SO4.And effect of temperature on inhibitor activity of PVTI was studied.The result shows that residual activity of PVTI was 70 percent after treatment at 100 ℃ for 60 min.It is indicated that PVTI is a protein with thermal stability.更多还原