【摘要】 利用设计的1对特异性引物,通过RT-PCR方法扩增出4株鸡传染性支气管炎病毒(IBV)安徽地方分离株膜蛋白M基因全长片段并进行了克隆测序。将各IBV安徽地方分离株与GenBank中注册的一些毒株M基因核苷酸序列及推导的氨基酸序列进行比较和系统进化关系分析,发现毒株间核苷酸序列同源性为88.5%~100%,其相应的氨基酸序列同源性为90.3%~100%;不同毒株间存着重组、缺失、插入及点突变等变异,从ATG至第140 bp区段的核苷酸序列变异频率最高;4株分离毒株属于同一个进化群的2个不同进化亚群,与我国常用疫苗毒株H120、M41和W 93不属同一个进化亚群。更多还原

【Abstract】 A pair of primers were designed and synthesized according to the published matrix(M) protein gene sequences of infectious bronchitis virus(IBV).The full lengths of M genes of 4 strains of IBV isolated from Anhui were amplified by RT-PCR.The amplified products were cloned and sequenced.The nucleotide sequences and the deduced amino acid sequences of M protein genes from the above isolates were compared with the published sequences of reference strains in GenBank,and the phylogenetic tree was constructed.The results showed that there were 88.5% to 100% of nucleotide sequence homology and 90.3% to 100%of deduced amino acid sequence homology among the strains.There were recombinations,deletions,insertions or mutations in the IBV isolates from Anhui.Furthermore,the fragment from start codon ATG to 140 bp showed the most variation;on the basis of the phylogenetic tree of M gene,it appeared that the IBV isolates in Anhui could be divided into two genetic subgroups,and were not in the same subgroup as the vaccine strains H120,M41 and W93 in china.更多还原