【摘要】 为了探索湖羊多胎分子机理,筛选影响湖羊多胎性状的特有候选基因,本试验选取16只经产湖羊母羊,分为单羔组和多羔组,同期发情后屠宰观察卵巢排卵数,并利用RT-PCR和荧光定量PCR技术对湖羊BMPRIA和BMPRⅡ基因的组织表达特征以及在单羔组和多羔组卵巢组织中的表达水平进行分析。结果表明:BMPRIA和BMPRⅡ基因除了在湖羊卵巢组织中有表达外,在下丘脑、垂体、子宫、心脏、肝脏、脾脏、肺脏、肾脏、肌肉、输卵管等组织内也均有表达;单羔组和多羔组间卵巢组织BMPRIA基因mRNA表达水平差异不显著(P>0.05),且与排卵数间没有显著相关性(P>0.05);但是,多羔组卵巢组织BMPRⅡ基因mRNA表达水平显著高于单羔组(P<0.05),且与排卵数呈显著正相关(r=0.722,P<0.05)。结果说明BMPRⅡ基因可能对湖羊排卵数起作用,是影响湖羊排卵数的候选基因。更多还原

【Abstract】 In order to understand the molecular mechanism and the candidate genes for high fecundity in Hu sheep,sixteen adult Hu ewes,grouped into single lambling(S) and multiple lambling(M) groups,were treated for synchronized estrous and sacrificed for tissue sampling and recording ovulation number.The tissue distributions and expression levels in the ovary for BMPRIA and BMPRⅡ mRNA in Hu sheep were investigated by RT-PCR and real-time PCR.The results showed that both the BMPRIA and BMPRII mRNA expressed in the ovary and other tissues,including hypothalamus,pituitary,uterus,heart,liver,spleen,lung,kidney,muscle and oviduct.There was no significant difference in the BMPRIA mRNA abundance in the ovary between S and M groups(P>0.05)and no correlation with ovulation number(P>0.05),but the BMPRⅡ mRNA abundance in the ovary was higher in M group than that in S group(P<0.05)and positively correlated with ovulation number(r=0.722,P<0.05).The results suggested that BMPRII might play an important role on ovulation number and might be a candidate gene for high fecundity in Hu sheep.更多还原