【摘要】 为了快速检测并鉴别鸡舍内外空气中的新城疫病毒(NDV),应用AGI-30气体收集器在一大型商品肉鸡舍舍内外收集气体,同时采集鸡的气管和泄殖腔拭子,采用兼并引物RT-PCR检测气体样品和拭子中的NDV,并鉴别NDV的毒力;同时分离、纯化气体样品中的NDV,用常规毒力学试验鉴别分离株的毒力。结果RT-PCR检测到舍内2/15的气体样品和4/15的拭子中同时有强毒和弱毒株,3/15气体样品和13/15的拭子只有弱毒;舍外上风向5 m处气体样品检测结果均为阴性,舍外下风向5 m处1/3的样品只有弱毒;其他样品均为阴性;从RT-PCR检测为阳性的样品中分离到3株强毒和4株弱毒株;从RT-PCR检测为阴性的样品中均未分离到NDV。结果表明兼并引物RT-PCR可直接、快速对气体样品进行检测及鉴别诊断。更多还原

【Abstract】 In order to rapidly detect and identify airborne Newcastle disease virus(NDV) in the indoor air and outdoor air of large-scale commercial broiler house,international standard air collector-AGI-30 was used to collect the air,Oropharyngeal and cloacal swabs were also collected.NDVs in the air samples and in the swaps were detected and virulence identified by degenerate primers based RT-PCR;at the same time NDVs in the samples were isolated and purified and then were virulence identified by conventional biological pathogenicity methods.The results showed that both virulent virus and avirulent virus could be detected simultaneously in 2/ 15 indoor air samples and 4/15 swaps,avirulent virus could be singly detected in 3/15 indoor air samples and 13/15 swaps,no virus could be detected in the air of 5 m upwind samples,avirulent virus could be singly detected in 1/3 samples of 5 m downwind air,no virus could be detected in all the other samples by degenerate primers based RT-PCR.Three virulent NDV and four avirulent NDV were isolated and virulence identified in the RT-PCR positive samples and no virus was isolated from the RT-PCR negative samples.The results suggest that degenerate primers based RT-PCR can detect and virulence identify NDV directly and rapidly.更多还原