【摘要】 初生仔猪泻痢的病原菌主要是肠毒素大肠杆菌(ETEC)F4,仔猪小肠上皮细胞有无F4受体对于仔猪抗病性具有重要意义。本试验采用δ差异显示法筛选仔猪F4ad受体相关基因。将获得的8条ESTs通过GenBank进行序列同源比对,发现其中1条与人糖基磷脂酰肌醇锚1(GPI1)基因有91%同源性。表明该基因可作为F4ad受体候选基因进行进一步研究。更多还原

【Abstract】 Enterotoxigenic Escherichia coli(ETEC) F4 was one of the most serious pathogenic bacteria inducing piglet diarrhea,ETEC F4 receptor in small intestine epithelium cells is of great importance for piglet′s disease-resistance breeding.Differential display reverse transcription(DDRT) PCR method was used to screen genes related to piglet ETEC F4 receptor in this study,8 ESTs were compared with GenBank.The result showed that one of them had 91% homologous to human glycosylphosphatidylinositol anchlor 1(GPI 1),which indicated that the GPI 1 gene might be the candidate gene of F4ad receptor.更多还原