【摘要】 通过扩增102头西藏小型猪以及16头巴马小型猪、17头贵州香猪的线粒体DNA控制区,测序并与国内其他猪种进行比较,研究西藏小型猪的遗传标记以及与其他国内地方猪种的亲缘关系。结果显示,西藏小型猪线粒体DNA D-loop区分3个区域。串联重复序列区处于中间位置,包含有15~29个10 bp的重复片段,分为A、B 2种类型。3′端340 bp,与国内其他猪种的序列相同,比较保守;D-loop 5′端704 bp,共有22个变异位点。由22个变异位点中归纳出25个单倍型,其中有2种主要的单倍型,分别占34.4%和36.6%。根据3个转换位点:305、500、691,将西藏小型猪分成了2组,几乎与串联重复序列所分的A、B 2组类型相对应。与西藏小型猪相比,巴马小型猪和贵州香猪D-loop 5′端变异位点较少,分别只有4种和2种单倍型,串联重复区也只有1个类型。说明西藏小型猪可能有2个母系祖先,并且与我国西南地区的猪种有较近的亲缘关系;不同的串联重复片段类型和5′端的变异位点可以联合组建西藏小型猪的遗传标记。更多还原

【Abstract】 To analyze mitochondrial DNA D-loop region sequences of Tibet Mini-pigs,and to explore their cytoplasmic DNA markers used to identify them and the phylogenetic relationship between Tibet Mini-pigs and other local pig breeds in China,mtDNA D-loop region in 102 Tibet Mini-pigs,16 Bama miniature pigs and 17 Guizhou Xiang pigs were amplified and sequenced,followed by comparing with the data of other Chinese domestic pigs.The result showed that the D-loop region of Tibet Mini-pigs was divided into three areas.The tandem repeat sequence which had 15-29 10 bp repeat motives and located in the middle of mtDNA D-loop region consisting of type A and B.340 bp sequence at 3′ end of the D-loop which was very conservative exhibited particularly high homology with the same region of other domestic pigs.704 bp sequence at 5′ end of the D-loop had 22 polymorphic sites.22 polymorphic sites deduced 25 haplotypes,among which the percentage of two major haplotypes reached 34.4% and 36.6%,respectively.Based on three transform sites(305,500,691),Tibet Mini-pigs were divided into two groups,nearly corresponding to type A and B of tandem repeat sequence.Compared with Tibet Mini-pigs,Bama miniature pigs and Guizhou Xiang pigs had four and two haplotypes,respectively,and had type A of tandem repeat sequence.It is inferred that Tibet Mini-pigs originated from two maternal ancestors and were close relative relationship with local pigs in southwest China.The repeating motif in combination with the variable sites at the 5′end of mtDNA D-loop region can be uesd as the genetic marker to identify Tibet Mini-pigs.更多还原