【摘要】 应用聚合酶链式反应-单链构象多态性(PCR-SSCP)的方法,以心脏-脂肪酸结合蛋白基因(H-FABP)为侯选基因,对6周龄北京鸭的3个群体(区分公母)共300个个体进行SNP分析。结果发现H-FABP第3内含子以本试验采用的引物碱基为第一碱基的第156碱基处发生1个C/G突变,此突变对Z4群体胴体质量、皮脂质量、皮脂率和全净膛质量有显著影响,并且对Z4(公)群体中胸肌质量、腿肌质量以及骨架质量有显著影响,因此可以将心脏-脂肪酸结合蛋白基因作为影响北京鸭Z4群体屠体性状的侯选基因。更多还原

【Abstract】 In this paper,300 Peking ducks were used to analyze H-FABP gene polymorphism by polymerase chain reaction single-strand conformation polymorphism(PCR-SSCP).The results showed that the polymorphism of the 3rd intron was caused by C/G mutation at 156 bp.In three populations(males and females),only the Z4 population resulted in genotypes which showed a significant difference in carcass weight,abdominal fat weight and eviscerated weight(P<0.05),the Z4(male) population resulted in genotypes which showed a significant difference in breast weight,leg weight,and skeleton weight.It implied that H-FABP could have a major effect on carcass performance or could be linked to genes that significantly affect carcass performance in Z4 population of Peking duck.更多还原