【摘要】 作者对O型口蹄疫病毒(FMDV)VP1双拷贝T细胞表位(aa 21-40)、B细胞表位(aa 141-160)融合蛋白2020-2020VP1及其与肠毒素大肠杆菌肠毒素融合后蛋白2020-B-2020和2020-B-2020-STI进行了免疫分析。动物试验表明,用2020-2020VP1、2020-B-2020和2020-B-2020-STI 3种融合蛋白分别免疫动物,免疫动物血清中均可产生针对FMDV的抗体。免疫豚鼠在低浓度FMDV刺激下能够产生特异性T淋巴细胞增殖反应,说明3种融合蛋白都能诱导机体产生FMDV特异性细胞及体液免疫反应。2020-B-2020和2020-B-2020-STI融合蛋白免疫雌鼠能够抵抗大肠杆菌强毒株攻击,免疫保护率如下:2020-B-2020 60%/1.5MLD,2020-B-2020-STI 100%/1.5MLD。2020-B-2020-STI免疫血清中具有STI中和抗体,且融合蛋白不具STI毒性。ELISA结果显示,2020-B-2020和2020-B-2020-STI能与霍乱毒素(cholera toxin)CTB抗体特异性结合。结果表明,2020-B-2020-STI具有开发成为口蹄疫及肠毒素大肠杆菌疫苗的应用价值。更多还原

【Abstract】 In this study,the fusion proteins including two copies of T-cell epitope(aa21-40) and B-cell epitope(aa141-160) of VP1 of food-and-mouth disease virus typed O and labile enterotoxin B subunit(LTB) and/not stable enterotoxinⅠ(STI) of Escherichia coli were analyzed as candidate vaccine against FMDV and E.coli.The proteins 2020-2020VP1,2020-B-2020 and 2020-B-2020-STI were used to vaccinate guinea pigs,rabbits and 6-8 weeks old BALB/c mice,then the immune responses were observed.The fusion proteins could induce proliferation of spleen T cells in vaccinated guinea pigs,and could elicit FMDV neutralizing antibody.It could be concluded that the three fusion proteins could activate FMDV-special cellular and humoral immune-response simultaneously.At the same time,the fusion proteins 2020-B-2020 and 2020-B-2020-STI could protect mice from challenge of enterotoxigenic E.coli C83902 and the protecting-rate were 60%/1.5 MLD and 100%/1.5 MLD respectively.The fusion protein including STI enterotoxin could induce neutralizing antibody for STI toxin in rabbits,moreover,the fusion protein had no STI toxicity.The fusion proteins 2020-B-2020 and 2020-B-2020-STI could be recognized specially by CTB antibody in ELISA.So that,it can be concluded that the fusion protein 2020-B-2020-STI is potent to be efficient FMDV and ETEC vaccine.更多还原