【摘要】 采用连续腹腔注射固定体积不同浓度梯度三氯化铝法,建立不同程度的鸡亚慢性铝中毒模型,测定雏鸡脾脏和法氏囊生长指数并应用光镜和透射电镜技术观察其结构。结果表明:(1)铝中毒组雏鸡脾脏和法氏囊生长指数均显著(P<0.05)或极显著(P<0.01)低于对照组,且存在剂量-效应关系;(2)光镜下,中、低剂量染铝组无明显病理变化,高剂量染铝组,脾脏出现凋亡,法氏囊小结生发中心增生;(3)电镜下,低剂量染铝组无明显病理变化,中、高剂量染铝组,脾脏淋巴细胞核膜破裂,线粒体肿胀、嵴断裂或消失,粗面内质网肿胀,网池增宽,融合成空泡状,出现典型的凋亡形态;法氏囊淋巴细胞核膜破裂,核溶解,有髓样小体形成,胞质内细胞器明显减少。上述结果说明,铝中毒严重抑制雏鸡脾脏和法氏囊生长发育,并对其造成明显的病理损伤。更多还原

【Abstract】 Model of chickens with deuto-chronicity different level of AI(Aluminium intoxication) toxic was established by continuous peritoneal injection of fixed volume and different concentration gradient aluminium trichloride.The growth index of spleen and bursa of Fabricius were detected,and the structure of them were observed by using light and electron microscope.The results showed that:(1)The growth index of spleen and bursa of Fabricius were significantly lower in chickens with AI toxic than that in the control ones and there were dose-effect relationship with aluminium trichloride.(2)Histopathologically,middle and low dose group had no apparent pathological change,in high dose group,spleen presented changes of apoptosis morphology and germinal center of folliculus lymphaticus in bursa of Fabricius hyperplasyed.(3)Ultrastructurally,low dose group had no apparently pathological change,in high dose group,karyolemma broke,mitochondria swelled,cristae broke or disappeared,rough endoplasmic reticulums dilated,cisternas widen,confluenced to vacuole and presented typical mark shape of apoptosis in lymphocyte of spleen and karyolemma broke,caryolysised,myelin bodys appeared,cell organs of intracytoplasm decreased obviously in lymphocyte of bursa of Fabricius.The results indicated that AI toxic greatly inhibited the development of spleen and bursa of Fabricius,and caused obvious pathological injury in these tissues.更多还原