【摘要】 选择年龄、体质量相近的杂交母羊(萨福克♂×小尾寒羊♀)24只,随机分为3组,分别饲喂不同蛋白水平的日粮(中蛋白日粮,低蛋白日粮和高蛋白日粮)。采用半定量RT-PCR方法,检测日粮不同蛋白水平对绵羊脂肪和肌肉中IGF-Ⅰ基因表达的影响。结果表明:日粮的蛋白质水平影响绵羊腹部皮下脂肪、肠系膜脂肪和半腱肌组织IGF-Ⅰ基因的表达,随着日粮蛋白质水平的升高IGF-Ⅰ表达量增加。但表达具有组织特异性,在腹部皮下脂肪、肠系膜脂肪中,中蛋白组和高蛋白组均显著高于低蛋白组(P<0.05),在半腱肌组织中,高蛋白组极显著高于低蛋白组(P<0.01),而中蛋白组与高蛋白组和低蛋白组间均无显著差异(P>0.05)。更多还原

【Abstract】 Twenty four crossbred ewes(Black-Suffolk♂× Small Tail Han sheep♀) were randomly allocated into three treatments with different dietary CP levels(high protein,medium protein and low protein) respectively.The expression level of IGF-Ⅰ mRNA in adipose tissue and muscle of sheep were quantified by RT-PCR.The results showed that expression of IGF-Ⅰ mRNA increased with the increase of dietary CP level in abdominal subcutaneous adipose tissue,mesentery adipose tissue and semitendinosus muscle of sheep.The effects of dietary CP level on IGF-Ⅰgene expression showed tissue specificity.For abdominal and mesentery adipose tissues,IGF-Ⅰ mRNA expression increased with the increase of dietary CP level(P<0.05),while the expression of IGF-ⅠmRNA in semitendinous muscle was significantly elevated only when the high protein diet was fed(P<0.01) and the difference of IGF-ⅠmRNA level between the medium and high protein treatments and between the medium and low protein treatments was insignificant(P>0.05).更多还原