【摘要】 为揭示脂滴表面蛋白Perilipin(PLIN)基因的发育性变化对家禽脂肪性状发育的作用,采用定量PCR方法首次测定1、14、28、42、56、70和84 d四川山地乌骨鸡胸肌、肝脏、腹脂和皮脂组织中PLIN基因的表达量,同时测定各个体活体质量、冠质量、皮脂厚以及肌内脂肪含量。结果,除28和70 d外,在各时间点不同组织间PLIN基因的表达量呈现腹脂>皮脂>胸肌>肝脏的规律,并且PLIN基因在鸡腹脂和皮脂中的表达量随个体日龄的增长明显上升。腹脂中PLIN基因的发育性变化同皮脂厚的发育性变化呈极显著正相关(P≤0.01),胸肌中PLIN基因的发育性变化同肌内脂肪含量的发育性变化呈极显著正相关(P≤0.01)。因此,推测PLIN基因在鸡脂肪性状形成中具有极重要的调控作用。更多还原

【Abstract】 In order to discover the function and mechanism that Perilipin(PLIN) gene acted on fatness traits of chicken,we adopted the real-time PCR method to test the relative expression quantity of PLIN gene in breast muscle,liver,abdominal fat and subcutaneous fat of Sichuan Mountainous Black-bone chicken at 7 growth points,including 1,14,28,42,56,70 and 84 day.We also tested each bird′s comb weight,living weight,subcutaneous fat thickness and intramuscular fat content in breast muscle.The results showed that the order of the relative expression quantity of PLIN gene in four tissues from high to low was abdominal fat,subcutaneous fat,breast muscle,and liver for all the growth points,except for 28 and 70 day.And the relative expression quantity of PLIN gene in abdominal fat and subcutaneous fat were increasing along with the growth of bird.Furthermore,the developmental variations of relative expression quantity of PLIN gene in abdominal fat were positively related with those of subcutaneous fat thickness(P≤0.01).And the developmental variations of relative expression quantity of PLIN gene in breast muscle were positively related with those of intramuscular fat content(P≤0.01).We inferred PLIN gene was a key regulator of the fatness traits development in chicken.更多还原