【摘要】 母猪怀孕后33~61 d期间,是胎猪卵母细胞发生的关键阶段。为掌握这一时期卵巢上组织细胞的发育变化,试验采集妊娠33、40、46、546、1 d的雌性胎猪卵巢进行苏木精—伊红染色。结果表明,妊娠33 d胎猪卵巢表面上皮由1~2层细胞构成,实质见到卵原细胞和髓质索结构;妊娠40 d时出现卵原细胞共质体结构和前细线期卵母细胞,髓质索状结构断裂;妊娠46 d共质体样结构增多,卵母细胞进入前细线期和细线期。髓质索断裂成片段;妊娠54 d时表面上皮由单层细胞构成,卵巢皮、髓可分,皮质中层见到合胞体样卵母细胞群,髓质相邻的皮质深层有一些原始卵泡正在形成;妊娠61 d胎猪卵巢皮、髓界限清晰,皮质中层是大量合胞体样卵母细胞群,皮质深层出现了较多的原始卵泡,髓质中卵泡结构退化,卵母细胞发生核固缩。更多还原

【Abstract】 From 33 to 61 day during gestation is critical stage of oogenesis for fetal pigs.To elucidate the histological change of ovaries in fetal pig,ovaries of 33,40,46,54,61 day-old fetal pigs were collected and H-E stained.The results showed that oogonia and medullary cords could be seen in 33 day-old fetal ovaries,which were coated by one or two layers of coelomic epithelial.Synpliasma-like cells and oocytes at preleptotene or leptotene occurred in 40 d fetal ovaries.Synpliasma-like cells increased and oocytes entered preleptotene or leptotene in 46 d fetal ovaries,when medullary cords separate into fragments.Superficial epithelium in 54 d fetal ovaries was made of one layer cells and boundary between cortex and medulla was differentiable.Syncytium-like cell groups appeared in middle cortex and primordial follicles appeared in deep cortex.The cortex and medulla in 61 d fetal ovaries was distinguished.The syncytium-like cell groups occurred in middle cortex and primordial follicles occurred in deep cortex.In medulla,however,follicles degenerated and oocytes had nucleus pyknosis.更多还原