【摘要】 以Novo435脂肪酶作为生物催化剂,叔丁醇作为反应溶剂,酶促葡萄糖与丙二酸和苹果酸的酯化反应,分别合成了丙二酸葡萄糖酯和苹果酸葡萄糖酯。反应产物的电喷雾(ESI)一级质谱分析和高效液相色谱质谱联用(LC/MS)分析表明:在No-vo435脂肪酶的催化作用下,丙二酸和苹果酸均可以与葡萄糖发生酯化反应,生成葡萄糖酯,且产物以单酯为主,含有少量二酯。反应产物的热裂解分析结果表明:所合成二元羧酸葡萄糖酯的热裂解产物多为醛、酮、酸、酯类小分子挥发性香味化合物。产物的烟草加香作用评价结果表明:添加二元羧酸葡萄糖酯后,卷烟的香气量显著提高,杂气明显减少,烟气细腻柔和程度也有一定改善,可见二元羧酸葡萄糖酯在烟草应用方面具有良好的应用前景。更多还原

【Abstract】 Using Novo435 lipase as a biological catalyst,tert-butanol as an organic solvent,enzyme-catalyzed synthesis of glucose-malonates from glucose and propanedioic acid,or glucose and malic acid were studied.After the vacuum distillation and purification,the white crystal products were prepared.This product was identified qualitatively by utilizing a variety of analytical techniques,such as ESI,as well as HPLC/MS.The analysis results showed that the product was a single-ester-based mixture,containing a small amount of di-ester.In addition,the composition of degradation products were studied by CDS/GC/MS,and the functions of identified degradation were briefly discussed.An expert panel investigated the effects of the product in cigarette,the results showed when the dosage was in 5～10 μg/g,the glucose esters could improve and smooth the flavour quality of cigarette,and reduce irritancy greatly,etc.更多还原