【摘要】 目的:探讨抗环瓜氨酸肽(CCP)抗体的检测在类风湿关节炎(RA)诊断中的价值。方法:采用酶联免疫吸附法(ELISA法)、间接免疫荧光法、速率散射比浊法分别检测RA组202例、非RA组(其他风湿病患者)103例和正常对照组(正常献血人员)20例的抗CCP抗体、抗角蛋白抗体(AKA)和类风湿因子(RF)。结果:RA组抗CCP抗体的阳性率为82.67%(167/202),非RA组为3.83%(4/103),正常对照组为0%(0/20)。抗CCP抗体对RA诊断的敏感性和特异性分别为82.67%、94.17%,RF对RA诊断的敏感性和特异性分别为86.63%、71.84%,AKA对RA诊断的敏感性和特异性分别为8.91%、99.03%。在RA患者中抗CCP抗体与AKA阳性率比较差异有统计学意义(P<0.01),抗CCP抗体与RF比较差异无统计学意义(P>0.05),3种方法的检测结果间存在相关性。结论:用ELISA法检测血清中抗CCP抗体简便、结果可靠,对RA诊断具有高度的敏感性和特异性。更多还原

【Abstract】 Objective:To detect anti-cyclic citrullianted peptide (CCP) antibody in patients with rheumatoid arthritis (RA) and explore its clinical value.Methods:Enzyme linked immunosorbent assay (ELISA),indirect immuneofluorescence (IIF),rate nephelometry were used to detect anti-CCP antibodies,Anti-keratin antibodies (AKA) and Rheumatoid factor (RF) in patients with 202 RA,103 non-RA other rheumatic cases and 20 normal donors group.Results:Among 202 patients with RA,167patients showed positive for anti-CCP (82.67%),whereas only 3.83% in 103 non-RA group.The sensitivity and specificity of anti-CCP antibodies were 82.67%,94.17% in RA respectively.The sensitivity and specificity of RF were 86.63%,71.84% in RA respectively.The sensitivity and specificity of AKA were 8.9%,99.03% in RA respectively.Anti-CCP antibodies were more sensitive and specific than RF and AKA for diagnosing RA,although there were correlations among three kinds of antibodies.Conclusion:Detecting anti-CCP antibodies by/ELISA may be a brief,standard and reliable method.Anti-CCP antibodies can be considered as one kind of masker antibody for RA.更多还原